Analysis Note

Positive ControlA2058 cells

Application

Cell Inhibition Assays (1-5 µg/ml, see application references)r>Flow Cytometry (1-5 µg/ml)r>Immunoblotting (not recommended)r>Immunofluorescence (1-5 µg/ml)r>Immunoprecipitation (1-2 µg/sample)r>Paraffin Sections (not recommended)

General description

This Anti-Integrin α₃ Mouse mAb (P1B5) is validated for use in Cell Inhibition Assays, FC, IF, IP for the detection of Integrin α₃.

Recognizes the α₃-subunit of the α₃/β₁ integrin complex protein in human mononuclear cells.

Purified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.

Immunogen

human mononuclear cells

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Will partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.

Albelda, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.Cheresh, D.A. et al. 1990. Cell57, 59.Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823.Vogel, B, et al. 1990. J. Biol. Chem.265, 5934.Wayner, E.A. et al.1988 J. Cell Biol.107, 1881.Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179. Hynes, R.O. 1987 Cell48, 549.Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.

Warning

Toxicity: Standard Handling (A)